In order to evaluate the application potential of the volatile compound benzothiazole in the control of gray mold, and to provide basis for further development and utilization, researchers from the College of Plant Protection of Shandong Agricultural University used 73 cases of Botrytis in Shandong Province to determine the pathogen of Botrytis. Cinerea) sensitivity to benzothiazole and comparison of different regions and different phenotype strains (carbendazim resistant and susceptible strains: CarR, CarS; procymidone resistant and susceptible strains: PrcR, PrcS; pyrimethanil Resistant and sensitive strains: PyrR, PyrS; bismuth amide resistance and sensitive strains: BosR, BosS) sensitivity difference; determination of benzothiazole and carbendazim, boscalid in the indoor mixing of Botrytis cinerea The inhibitory effect of benzothiazole on cucumber gray mold was verified by in vitro cucumber leaf inoculation test, and the effect of different fumigation dose and time on the pathogenicity of Botrytis cinerea was observed. The benzothiazole was observed by scanning electron microscope. The effect on the morphology of gray mold.
The results showed that the EC50 value of benzothiazole against Botrytis cinerea was 0.38~1.08 μL/L, and the mean value was 0.62 μL/L. There was no sensitivity difference between different regions; carbendazim and procymidone in Shandong area Pyrimethanil, boscalid resistance and sensitive Botrytis cinerea were sensitive to benzothiazole, indicating that these four fungicides have no cross-resistance with benzothiazole; in the range of 0.1 to 3 μL/L of benzothiazole The hyphae are more sensitive than spores; the inhibition rate of mycelium by 2 μL/L benzothiazole is as high as 86.12%, the inhibition rate on the elongation of the tube is 55.15%, and the inhibition rate on spore germination is only 19.30%. . It can be seen that the sensitivity of the compounds to different growth stages is different. The combination of benzothiazole and carbendazim has no synergistic effect or additive effect on the control of cucumber gray mold. When the two are mixed, the main antibacterial effect of the agent is related to the concentration and proportion of each component used in the mixture; benzothiazole There is a significant synergistic effect with the combination of boscalid, and the practical application methods, effects and synergistic mechanisms in the field need to be further clarified. The in vitro leaf test showed that benzothiazole had both protection and treatment control effect against cucumber gray mold. The therapeutic effect of 2 μL/L was 91.01%, which was higher than the control agent boscalid 300 mg/L (78.90%). And significantly higher than 48.25% protection against effects. Benzothiazole can significantly reduce the pathogenicity of Botrytis cinerea hyphae, and the degree of inhibition is positively correlated with the concentration of benzothiazole. Scanning electron microscopy showed that benzothiazole could make the mycelium abnormal, the hyphae dried up, the branches increased, and the surface was sunken.
The researchers believe that benzothiazole has high virulence to Botrytis cinerea in different parts of Shandong Province and is suitable for use as a fumigant for the control of gray mold. At the same time, benzothiazole has a broad spectrum of action, and is effective against Sclerotinia sclerotiorum, rice sheath blight, red stalk, scutellaria, root-knot nematode, and potato rot nematode, and has a large field of plant protection. Application potential.
(Source: Pesticide Newsletter)
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