technical background
Oligosaccharides can effectively regulate micro-ecological balance, reduce morbidity, improve animal health and production performance, and are widely used antibiotic substitutes, such as oligofructose, soybean oligosaccharide, and mannan oligosaccharide oligosaccharides.
Oligofructose is a pollution-free and functional dual-effect feed additive with many excellent biological and physiological effects: 1. Stimulates the growth of bifidobacteria, inhibits the growth of pathogenic bacteria causing diarrhea; 2. Combines with specific glycosylation of intestinal pathogens The combination of sites allows the pathogen to lose or reduce the chance of specifically recognizing and binding with the intestinal wall of the host animal, reducing the colonization and reproduction of Salmonella, Escherichia coli and Enterococcus in the intestine; 3. Inducing microbial polysaccharides in the digestive tract of the animal Production of carbohydrate hydrolase; 4. Direct or indirect positive stimulation of immune activity in animals.
The project is a project supported by the National High Technology Research and Development Program (863 Program) and the National Natural Science Foundation of China.
Technological advancement
1. One-step enzymatic degradation or transfer synthesis to produce oligofructose, the production process is simple.
2, two processes, two enzymes, two substrates, the same product, industrialization entry point.
3. The fermentation level of the restricted key enzyme is increased, and the production cost is lowered.
4, the product active ingredient content (purity) is high, the application effect is significant, reducing the use of conventional antibiotics; improving the safety and security of livestock and poultry products.
5. As a functional feed additive, oligomeric oligosaccharides have the characteristics of stability, safety, and no toxic side effects.
Technological innovation
1. The special measures of excessive substrate concentration can make the yield of oligofructose reach 69%, and the breakthrough can not exceed the theoretical limit of 50%. The increase of this yield figure is of great significance in the field of industrialization.
2, the use of low-dose enzymes can obtain a high ratio of canetriose (56% of the total product, accounting for 82% of total oligosaccharides), and canetriose is one of the best members of oligofructose, The purification cost of oligofructose can be significantly reduced.
3. The fructose transferase produced has high catalytic activity. In the oligofructose synthesis process, the ratio of enzyme activity to substrate mass is only 0.6:1 (U/g), which greatly reduces the cost of enzyme use.
4, the production of fructose transferase strain enzyme production amount of 250U / ml fermentation broth, reducing the cost of enzyme production.
5. The time to reach the highest enzyme level is shortened by 50 hours.
6, the only key enzyme production technology of inulin oligofructose production is at the international leading level.
Application range
Mainly used in feed additives, it can significantly improve the feed conversion rate and the health of the animal body, replace some of the conventional antibiotics for feeding, reduce the antibiotic residue of animal products, improve the quality of animal products, and significantly increase the growth rate of animals.
Project prospects
At present, the production and use of oligofructose products in Asia, North America and Europe are on the rise. According to estimates by the National Feed Office, by 2005, China's compound feed was 120 million tons, and the demand for oligofructose was about 250,000 tons. With the development of the market, the demand for oligofructose in the feed industry and aquaculture industry at home and abroad will become larger and larger, and will increase at a rate of 10% per year. This indicates that the promotion prospect of oligofructose is very broad.
Production Process
Major equipment
Fermenter, reaction kettle, drying equipment, boiler
Investment strength estimate
The annual output of 1,000 tons requires about 10 million yuan.
Source: China Feed Additives 05, 02
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